Antibody Production and Purification Reagents and Kits
Antibody Production and Purification Reagents and Kits
Active Motif Sortag-IT™ ATTO 488 Labeling Kit
Sortag-IT Labeling Kits are designed for site-specific labeling of Active Motif's highly specific AbFlex recombinant antibodies (rAb) via the Sortase tag recognition sequence (LPXTG) that is incorporated into the heavy chains of each AbFlex antibody. Directly conjugate fluorophores, enzymes (HRP, AP), biotin, peptides, DNA, carbohydrates or other labels to the terminal end of the heavy chain to ensure the antigen binding site remains available for target recognition. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to provide a faster, more efficient labeling reaction. Simply add your AbFlex antibody with the poly-Glycine label, add Sortase A5 and incubate for approximately 1 hour. Purification […] For more information, please visit: https://www.activemotif.com/catalog/1230.
