Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, low ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
Thermo Scientific™ RapidFinder™ Salmonella species, Typhimurium and Enteritidis Multiplex Flex Kit
Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with the RapidFinder Salmonella species, Typhimurium and Enteritidis Multiplex Flex Kit.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
Thermo Scientific™ Verso 1-step RT-qPCR Mix, low ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 PCR Assay
Simply and accurately detect E. coli O157:H7 on beef meats, fruits and vegetables including large test portion sizes with the Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
SureTect™ Listeria Species PCR Assay
Rapidly detect Listeria species in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay
Rapidly detect Staphylococcus aureus in food and environmental samples with the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay.
Thermo Scientific™ SureTect ™ Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay
Rapidly detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR Assay.
Thermo Scientific™ ABsolute™ qPCR Mixes
ABsolute qPCR Mixes are optimized for use with hybridization probes.
Promotions are available
Precipio™ HemeScreen BCR/ABL1 Panel-20
The HemeScreen BCR/ABL1 Panel-20 is an RUO reagent set kit used to screen for 3 major BCR/ABL1 isoforms (p190, p210, and p230) and 1 minor isoform (p203) associated with BCR/ABL1-induced leukemogenesis, including CML and ALL using HRM methods.
Precipio™ HemeScreen CEBPA Kit-100
The HemeScreen CEBPA Kit-100 is an RUO reagent set kit used to detect CEBPA gene mutations through HRM methods.
Promega GoTaq™ Probe 2-Step RT-qPCR System
Robust system to simplify RNA expression analysis. The GoTaq™ Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels.