Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Thermo Scientific™ ABsolute™ qPCR Mixes
ABsolute qPCR Mixes are optimized for use with hybridization probes.
Thermo Scientific™ SureTect™ Listeria Species PCR Assay
Detect Listeria species in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Thermo Scientific™ Verso 1-step RT-qPCR Mix, low ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, low ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Thermo Scientific™ ROX Solution, 50 µM
ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.
Thermo Scientific™ SureTect™ Cronobacter Species PCR Assay
Rapidly detect Cronobacter species in powdered infant formula and production environment samples in less than 20 hours with minimal handling and maximum confidence in results.
Thermo Scientific™ SureTect™ Salmonella Species PCR Assay
Detect Salmonella species in even the most challenging food types with this multiplex real-time assay that provides highly-specific detection of the invA gene.
Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay
Detect Listeria monocytogenes in even the most challenging food types with this multiplex real-time assay that provides highly-specific detection of the prfA gene.
MP Biomedicals™ qPCR& Go SYBR No-ROX Kit
Fast and sensitive qPCR SYBR Master Mix. For all other platforms, e.g. Roche, Bio-Rad (not "e;i"e; porfolio), Qiagen, etc.
MP Biomedicals™ cDNA Synthesis & Go Kit
Provides a rapid and very sensitive method for first strand cDNA synthesis for use in real-time PCR studies
SPEX CertiPrep™ 2-Color Aspergillus Detection Kit
Provides a rapid and accurate test to determine the presence of Aspergillus DNA in a sample.
Streck ARM-D Kit, β-Lactamase (RUO) and Mix

Streck ARM-D™ Kits are comprehensive, cost-effective molecular kits that detectthe most clinically important β-lactamases for current and emerging threats.
Precipio™ HemeScreen IDH1 Kit-100
The HemeScreen IDH1 Kit-100 is an RUO reagent set kit used to detect IDH1 gene mutations through HRM methods.
Precipio™ HemeScreen NPM1 Kit-100
The HemeScreen NPM1 Kit-100 is an RUO reagent set kit used to detect NPM1 gene mutations through HRM methods.
Precipio™ HemeScreen CALR Exon 9 Kit-100
The HemeScreen CALR Exon 9 Kit-100 is an RUO reagent set kit used to detect CALR Exon 9 gene mutations through HRM methods.
Precipio™ HemeScreen AML Panel DNA Validation Set
The HemeScreen AML Panel DNA Validation Kit contains 20 blinded control samples and pre-plated AML panels.
Precipio™ HemeScreen MPL Exon 10 Kit-100
The HemeScreen MPL Exon 10 Kit-100 is an RUO reagent set kit used to detect MPL Exon 10 gene mutations through HRM methods.
Precipio™ HemeScreen BCR/ABL1 Panel-20
The HemeScreen BCR/ABL1 Panel-20 is an RUO reagent set kit used to screen for 3 major BCR/ABL1 isoforms (p190, p210, and p230) and 1 minor isoform (p203) associated with BCR/ABL1-induced leukemogenesis, including CML and ALL using HRM methods.
Precipio™ HemeScreen JAK2 Exon 13 Kit-100
The HemeScreen JAK2 Exon 13 Kit-100 is an RUO reagent set kit used to detect JAK2 Exon 13 gene mutations through HRM methods.
Precipio™ HemeScreen DNMT3A Kit-100
The HemeScreen DNMT3A Kit-100 is an RUO reagent set kit used to detect DNMT3A gene mutations through HRM methods.
Precipio™ HemeScreen CLL Limit of Detection Kit
The HemeScreen CLL Limit of Detection Kit contains five control vials to verify the lower level of detection for the HemeScreen Cytopenia panel genes: MYD88, NOTCH1, CXCR4, and SF3B1.
Precipio™ HemeScreen CLL Panel-3
The HemeScreen CLL Panel-3 is an RUO pre-plated kit used to detect CLL-related gene mutations for MYD88, NOTCH1, CXCR4, and SF3B1 genes through HRM methods.
Precipio™ HemeScreen MYD88 Kit-100
The HemeScreen MYD88 Kit-100 is an RUO reagent set kit used to detect MYD88 gene mutations through HRM methods.
Precipio™ HemeScreen ASXL1 Kit-100
The HemeScreen ASXL1 Kit-100 is an RUO reagent set kit used to detect ASXL1 gene mutations through HRM methods.
Precipio™ HemeScreen TERT Kit-100
The HemeScreen TERT Kit-100 is an RUO reagent set kit used to detect TERT gene mutations through HRM methods.