Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price

MilliporeSigma™ Immobilon™-FL PVDF Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.

MilliporeSigma™ Immobilon™ Blotting Filter Paper
Chromatography-grade blotting filter paper precut to the most popular Western blotting sizes

MilliporeSigma™ Calbiochem™ TMB, Insoluble
Peroxidase substrate which produces stable, insoluble, aquamarine blue precipitate at reaction site.
MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
MilliporeSigma™ Novagen™ 5% Alkali - Soluble Casein
Used as blocking reagent for Western blot applications.
MilliporeSigma™ Calbiochem™ Protein G, Peroxidase Conjugate
Purified recombinant protein G conjugated to peroxidase