Western Blot Products

Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.

The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.

Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.

Western Blot and Nucleic Acid Membranes and Filter Paper (51)

Western Blot Equipment and Supplies (19)

Western Blot Substrates and Substrate Kits (16)

Antibody-Binding Proteins and Conjugates (11)

General Western Blot Reagents (8)

Protein Method Blocking Buffers (7)

Biotin-Binding Proteins and Conjugates (7)

Target-Specific Western Blot Kits (3)

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Material

PVDF (33)
Immobilon-P^SQ (7)
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0.45 μm (32)
0.2 μm (9)

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Sheet (21)
Roll (10)

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Dot/Slot Blot (6)
Immunoblot (Western) (5)
Western Blot (5)
Western Blotting (5)
Mass Spectrometry (2)
Cell Function Assay (1)
Collecting primary antibodies for reuse (1)
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MilliporeSigma™ Immobilon™-P PVDF Membrane

Hydrophobic PVDF Transfer Membrane for western blotting

Pricing & Availability
Specifications

Pore Size	0.45 μm
Material	PVDF

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Pricing & Availability
Specifications

Substrate	Western Chemiluminescent HRP
Content And Storage	2°C to 8°C
Shelf Life	9 Months
For Use With (Application)	Dot/Slot Blot

MilliporeSigma™ Immobilon™-FL PVDF Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.