Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Cytiva Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls
Use for robust protein blotting. Cytiva Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Sheets

Use for Western blots, dot-blot assays, and other protein or nucleic acid methods. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Sheets offer excellent sensitivity, resolution, and low background.

Cytiva Amersham™ Hyperfilm™ MP

Use in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers. Cytiva Life Sciences™ Amersham™ Hyperfilm™ MP is an excellent multipurpose film constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti scratch layer.

Cytiva Whatman™ Protran™ Nitrocellulose Blotting Membranes: 0.45μm BA85 Protran Circles

100% pure nitrocellulose to ensure the highest binding capacity possible

Cytiva Amersham™ Hybond™ -N+ Membranes

Use for the highest sensitivity in DNA and RNA blotting. Cytiva Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with radioactive and nonradioactive chemiluminescence and chemifluorescence detection.

Cytiva Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.
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Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva Whatman™ Gel Blotting Paper Grade GB003

A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots

Cytiva Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.