Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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Small and/or diverse supplier based on Federal laws and SBA requirements.
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With low endotoxin content
Dilution | 1 X |
---|---|
Product Type | DPBS |
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Formulation | 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide |
---|---|
Physical Form | Liquid |
pH | 7.7 |
Chemical Name or Material | Running Buffer |
Recommended Storage | Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C |
Concentration | 20X |
Product Line | NuPAGE |
Gibco™ DPBS (10X), no calcium, no magnesium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Dilution | 10 X |
Content And Storage | Store at room temperature. |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.4 |
Product Line | Ambion™ |
Boiling Point | 100°C |
---|---|
Name Note | 1X Solution, pH 7.4 |
CAS | 77-86-1 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
Synonym | TBS |
DNase | DNase free |
Recommended Storage | RT |
ChemAlert Storage Symbol | Gray |
Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1,7732-18-5,1185-53-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1,7732-18-5,1185-53-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Tris-HCl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Gibco™ DPBS, no calcium, no magnesium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Form | Liquid |
---|---|
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.3 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 270 - 300 mOsm/kg |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Thermo Scientific Chemicals Potassium phosphate, 0.5M buffer soln., pH 7.4
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
CAS | 7778-77-0 |
---|---|
Molecular Weight (g/mol) | 136.08 |
MDL Number | MFCD00011401 MFCD00147253 |
SMILES | [K+].OP(O)([O-])=O |
IUPAC Name | potassium dihydrogen phosphate |
InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
Molecular Formula | H2KO4P |
Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 6.8
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
PubChem CID | 79723 |
---|---|
CAS | 5625-37-6 |
Molecular Weight (g/mol) | 302.36 |
ChEBI | CHEBI:44933 |
MDL Number | MFCD00006159 |
SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O6S2 |
HEPES, cGMP Manufactured, 99.5%, Spectrum™ Chemical
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CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
CAS | 7365-45-9 |
---|---|
Molecular Weight (g/mol) | 238.30 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Formulation | 1X: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3 |
---|---|
Physical Form | Liquid |
pH | 7.3 |
Chemical Name or Material | Running Buffer |
Concentration | 20X |
Product Line | NuPAGE |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Gibco™ ACK Lysing Buffer
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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