Buffers and Standards
Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
Sodium thiosulfate, 0.01N Standardized Solution, Thermo Scientific Chemicals
CAS: 7772-98-7 Molecular Formula: Na2O3S2 Molecular Weight (g/mol): 158.10 MDL Number: MFCD00003499 InChI Key: AKHNMLFCWUSKQB-UHFFFAOYSA-L Synonym: sodium thiosulfate,sodium thiosulphate,disodium thiosulfate,sodium thiosulfate anhydrous,hypo,sodiumthiosulfate,chlorine cure,chlorine control,declor-it,thiosulfuric acid, disodium salt PubChem CID: 24477 IUPAC Name: disodium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane SMILES: [Na+].[Na+].[O-]S([S-])(=O)=O
Hydrogen peroxide, 35% w/w aq. soln., stab., Thermo Scientific Chemicals
CAS: 7722-84-1 Molecular Formula: H2O2 MDL Number: MFCD00011333 InChI Key: MHAJPDPJQMAIIY-UHFFFAOYSA-N Synonym: oxydol,perhydrol,superoxol,interox,hydrogen dioxide,inhibine,peroxaan,albone,hioxyl,kastone PubChem CID: 784 ChEBI: CHEBI:16240 IUPAC Name: hydrogen peroxide SMILES: OO
Sodium hydroxide, 0.1N Standardized Solution, Thermo Scientific Chemicals
CAS: 1310-73-2 Molecular Formula: HNaO Molecular Weight (g/mol): 39.997 MDL Number: MFCD00003548 InChI Key: HEMHJVSKTPXQMS-UHFFFAOYSA-M PubChem CID: 14798 ChEBI: CHEBI:32145 IUPAC Name: sodium;hydroxide SMILES: [OH-].[Na+]
Thermo Scientific Chemicals TRIS-glycine-SDS running buffer (10X), pH 8.3
TRIS-glycine-SDS running buffer (10X), suitable for protein gel analysis, is used in several research areas, including biochemistry, molecular biology, cell biology, and immunology.
Saponin permeating solution, 0.5% w/v soln. in PBS (10X), Thermo Scientific Chemicals
Cell permeabilizing agent
Thermo Scientific Chemicals MOPS, 99%, for biochemistry
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-Morpholino)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
Thermo Scientific Chemicals TAE (50X), TRIS + acetate + EDTA
TAE (50X), TRIS + acetate + EDTA is a buffer composed of Tris, acetate, and EDTA components and is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 9.5
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), RNase free
Helps to maintain a constant pH
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.5
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Thermo Scientific Chemicals HEPES, 99%
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Thermo Scientific Chemicals TBE running buffer (10X)
Commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels.
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
