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Vector Laboratories Lens Culinaris Agglutinin (LCA, LcH) Unconjugated 10 mg
Supplier: Vector Laboratories L104010
Lens culinaris agglutinin (LCA) contains two subunits of 17 kDa and two of 8 kDa. It has a narrower specificity than ConA because it recognizes sequences containing α-linked mannose residues and other sugars as part of the receptor structure. An α-linked fucose attached to the N-acetylchitobiose of the core oligosaccharide enhances affinity. The narrower specificity allows subfractionation of glycoproteins and -peptides after initial isolation with Con A. LCA prevents skin allograft rejection and can purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin. This unconjugated form is supplied as a solution preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
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