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Invitrogen™ TrueCut™ Cas9 Protein v2
A next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.
Supplier: Invitrogen™ A36498
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Description
TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aid delivery to the nucleus, increasing the rate of genomic DNA cleavage.- Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2x higher editing efficiency in difficult targets than the competition
- High quality—manufactured under strict ISO 13485 quality standards
- Validated protocols for large number of cell types to achieve success faster
- Transfection-ready, using either lipid-mediated transfection reagents or electroporation
- Eliminates time-consuming cloning steps
- Available in 2 concentrations: 1μg/μL for use in standard editing scenarios and 5μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
Certifications
CoA
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Specifications
5 μg/μL | |
Molecular Biology | |
NC_002737.2:854751-858857 | |
901176 | |
cas5, csn1 | |
Untagged | |
Genome Editing | |
Genome Editing |
20μL TrueCut Cas9 Protein v2 (5 mg/mL) | |
20 μL TrueCut Cas9 Protein v2 (5 mg/mL) Store at -5 to -30°C. |
|
E. coli | |
Spy_1046 | |
Recombinant | |
Dry Ice | |
Cas9 Protein v2 | |
Invitrogen™ |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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