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Edvotek™ Transformation of E. coli with Green and Blue Fluorescent Protein Plasmids Classroom Kit

Name: Edvotek&trade; Transformation of E. coli with Green and Blue Fluorescent Protein Plasmids Classroom Kit
Brand: Edvotek&trade;
SKU: S68653
Price: 172.00 USD

Helps students develop an understanding of bacterial transformation by plasmid DNA

Supplier: Edvotek™ 222

Catalog No. S68653

$172.00 / Each of 1

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Alert:

Transformation experiments contain antibiotics which are used for the selection of transformed bacteria. Students who have allergies to antibiotics such as penicilllin, ampicillin, kanamycin or tetracycine should not participate in this experiment. All components intended for educational research only. They are not to be used for diagnostic or drug purposes, nor administered to or consumed by humans or animals.

Includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ agar, Luria broth, petri dishes, sterile pipets and loops

Requires :

Automatic micropipet (5-50μL) and tips
2 water baths (37°C and 42°C)
Thermometer
Incubation oven (34°C and 37°C)
Pipet pumps or bulbs
Ice
Marking pens
Bunsen burner, hot plate or microwave oven
Hot gloves
Long-ave UV light (EDVOTEK No. 969 recommended)

Description

Enables students to observe the acquired phenotypic trait of green or blue fluorescent protein exhibited by transformed bacterial cells
Transformants acquire green (GFP) or blue (BFP) fluorescent protein genes contained in two different plasmids; the GFP gene was mutated to produce the blue protein
Transformed cells are selected based on antibiotic resistance
Colonies which express GFP or BFP protein are visibly green and pale blue and will fluoresce when exposed to long-wave UV light

Order Info

Class size: 10 experiments. Shipped ambient. Supercoiled pFluoroGreen™, Supercoiled pFluoroBlue™, ampicillin, and IPTG require -20°C storage. Note:Transformation cell slant should not be frozen.

Specifications

Product Type	Transformation of E. Coli Kit
Includes	Instructions, lyphocells, plasmid dna, iptg, ampicillin, transformation solution, readypour™ agar, luria broth, petri dishes, sterile pipets and loops
Class Size	10 Lab Groups

For Use With (Application)	Helps students develop an understanding of bacterial transformation by plasmid dna
Requires	Waterbath , 37°C incubation oven, microwave or hot plate, long wave UV lamp

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