Thermo Scientific™ TMT11-131C Label Reagent

Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry.

Supplier:  Thermo Scientific™ A58333

Catalog No. FERA58333

Description

Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT11-131C tag structure is identical to the other TMT10pex tags, but the TMT11-131C mass reporter is labeled with only 13C isotopes. The addition of the TMT11-131C tag enables separation of the 131-mass reporter ion of the original TMT10plex reagents into N and C variants, creating an 11th channel for relative quantitation using high resolution Orbitrap MS instruments.

The advantages of TMT label reagents include increased multiplexed relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents plus TMT11-131C is ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or to improve statistics on biological replicates.

Features of this TMT11-131C Label Reagent include:
• Multiplex—concurrent MS analysis of up to 11 samples derived from cells, tissues, or biological fluids
• Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
• Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible—optimized for use with high resolution Thermo Scientific Orbirtrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3

TMT11-131C is available as a standalone tag (Cat. No. A34807, A37724), as part of this TMT10plex Isobaric Label Reagent Set plus TMT11-131C, or as part of the complete 11plex set of reagents in a 96-well plate format. When combined with the industry-leading, high resolution Orbitrap instruments and software, TMT reagents provide integrated total solutions for quantitative protein expression analysis.

Specifications

• Description: TMT11plex Label Reagents ​in stabilized solution, ​96-well plate, 8 x 40 μg​ (per tag)
• For Use With (Equipment): Mass Spectrometer
• Product Line: TMT10plex™
• Label or Dye: Tandem Mass Tag™ (TMT) Labels
• Final Product Type: Protein PEPTIDE
• Workflow Step: Protein Labeling
• Form: Liquid

• Formulation: Liquid in stabilized DMSO solution
• Product Type: Isobaric Label Reagent Set
• Content And Storage:

  Sufficient for: Eight 11-plex (8 x 11-way) experiments
  • TMT11-126 Label Reagent, 8 x 40 μg
  • TMT11-127N Label Reagent, 8 x 40 μg
  • TMT11-127C Label Reagent, 8 x 40 μg
  • TMT11-128N Label Reagent, 8 x 40 μg
  • TMT11-128C Label Reagent, 8 x 40 μg
  • TMT11-129N Label Reagent, 8 x 40 μg
  • TMT11-129C Label Reagent, 8 x 40 μg
  • TMT11-130N Label Reagent, 8 x 40 μg
  • TMT11-130C Label Reagent, 8 x 40 μg
  • TMT11-131N Label Reagent, 8 x 40 μg
  • TMT11-131C Label Reagent, 8 x 40 μg
  
  Store at -20°C.

• Starting Material: Cell Lysate
• Detection Method: Mass Spectrometry
• Quantity: 88 Reactions