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Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0
Thermo Scientific offers a variety of accessories for instrumentation to keep labs operating efficiently.
$198.10 - $372.00
Catalog Number | Mfr. No. | Description | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Description | Price | Quantity & Availability | |||||
PI21020
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Thermo Scientific™
21020 |
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 |
Each for $198.10
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PI21012
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Thermo Scientific™
21012 |
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 |
Each for $372.00
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21020
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Thermo Scientific™
21020 |
0.25 in. x 4.6mm I.D. Frit | Item Discontinued This item has been discontinued by the supplier. Please Sign In to view product availability in your area. | N/A | |||||
Description
Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.
Highlights:
Three optimized binding buffers – for Protein A, Protein G or Protein A/G
Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)
Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose
Item-Specific Features:
Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)
Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide
Includes:
Kits contain both binding and elution buffers
Specifications
Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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