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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Supplier:  Thermo Scientific™ K0982

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Catalog No. FERK0982


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Includes: Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platfo

Description

Description

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Contains:
• 4 x 1.25 mL Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 500 x 20 μL reactions. The master mix contains fluorescein passive reference dye for specific real-time platforms.
• 4 x 1.25 mL Nuclease-Free Water

Store at -20°C.
High
Taq DNA Polymerase
BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
qPCR Master Mix
Gene Expression
2 X
DNA, dsDNA
SYBR
qPCR
Standard
Luminaris
500 Reactions
2X
500 Reactions
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