Applied Biosystems™ TaqMan™ RNase P Control Reagents Kit

TaqMan™ RNase P Control Reagents Kit

Supplier:  Applied Biosystems™ 4316844

Catalog No. 43-168-44

Description

The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems™ TaqMan™ RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan™ Universal PCR Master Mix using the provided human genomic DNA as template.

Pre-designed primers and TaqMan™ probe eliminate assay design

Easy-to-use primer and TaqMan™ probe mix minimizes assay set-up time

Designed with limiting primer concentrations to eliminate assay optimization

Rapid assay development guidelines can minimize optimization time

Comprehensive Guidelines Included

Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In

All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Calibration Type: Passive Internal Reference Standards
• Control Template: Human Genomic DNA
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Label or Dye: VIC
• Product Type: Real Time PCR Reagents
• Species: Human
• Target: RNase P

• Content And Storage: Control Primer-Probe Set(s) & Template(s)
• Format: Tube
• PCR Method: Multiplex qPCR
• Internal Probe Modification: TAMRA™ Quencher (3'), VIC™ (5')
• Product Line: TaqMan™
• Shipping Condition: Wet Ice
• Sufficient For: 1000 Reactions
• No. of Reactions: 1000 Reactions

For Research Use Only. Not for use in diagnostic procedures.