Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

Supplier:  Applied Biosystems™ 4488593

Catalog No. 44-885-93

Description

TaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Content And Storage: Contains one 5-mL bottle of 2X master mix.
  
  Store at 2–8°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Format: Bottle
• PCR Method: qPCR
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Product Type: PreAmp Master Mix
• For Use With (Application): Pre-amplification
• Sample Type: cDNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Product Line: TaqMan™
• Sufficient For: 200 Reactions
• No. of Reactions: 200 Reactions
• Volume: 5 mL

For Research Use Only. Not for use in diagnostic procedures.