Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

TaqMan™ hPSC Scorecard™ Kit, Fast 96-well

Supplier:  Applied Biosystems™ A15871

Catalog No. A15871

Description

The Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit 2 x 96w FAST enables verification of pluripotency and determination of lineage bias for both ES and iPS cell lines. Each of the two 96-well plates contains 94 predefined TaqMan™ Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is included for your convenience. To purchase the panel alone, see TaqMan™ hPSC Scorecard™ Panel 2 x 96w FAST. This product is also available in a 384-well format.

Assess an entire gene signature in one simple experiment

Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software

Identify lines with trilineage differentiation potential

Evaluate inductive differentiation specified to one germ layer

Access validated content for increased confidence in your results

Assess an Entire Gene Signature in One Experiment
The TaqMan™ hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan™ Master Mix to your cDNA of interest and transfer to the 96-well plate with a multichannel pipette.

Compare Results to a Reference Standard
The TaqMan™ hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems™ qRT-PCR systems and is available at no additional charge.

Identify Lines with Trilineage Differentiation Potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluate Inductive Differentiation Specified to One Germ Layer
The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

Access Validated Content
The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

What You Get
The TaqMan™ hPSC Scorecard™ Panel 2 x 96w FAST includes:

Two 96-well plates of 94 TaqMan™ Gene Expression assays

Two optical plate covers

Two 1 mL vials TaqMan™ Fast Advanced Master Mix

Specifications

• Content And Storage: • Two 96-well plates each containing the 96-assay panel (room temperature)
  • Two optical covers (room temperature storage)
  • Two 1 mL vials of TaqMan™ Fast Advanced Master Mix (thawed for shipping, DO NOT RE-FREEZE, store at 2–8°C)
• Form: Dried Down
• Reaction Speed: Fast
• Product Line: TaqMan™
• Species: Human
• No. of Reactions: 96 Reactions

• Detection Method: Primer-probe
• Format: 96-well plate
• For Use With (Equipment): QuantStudio™ 12k Flex, StepOnePlus™, Fast Mode, ViiA™ 7 System
• Research Category: Stem Cell Research
• For Use With (Application): Stem Cell Analysis

For Research Use Only. Not for use in diagnostic procedures.