Invitrogen™ SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

Supplier:  Invitrogen™ 12595100

Catalog No. 12-595-100

Includes:

• SuperScript IV RT Mix, 12.5µL
• 2X Platinum SuperFi RT-PCR Master Mix, 625µL
• Nuclease-free water, 1.25mL

Description

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

Specifications

• Content And Storage:

  • SuperScript IV RT Mix (50 μL)
  • 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
  • ezDNase Enzyme (100 μL)
  • 10X ezDNase Buffer (100 μL)
  • Nuclease-free water (2 x 1.25 mL)
  
  Store at –20°C.
  
  

• Format: Kit
• PCR Method: 1-step RT-PCR
• Reaction Speed: 10 min.
• Optimal Reaction Temperature: 45°C to 60°C
• Ribonuclease H Activity: Reduced
• Final Product Type: PCR Amplified cDNA
• No. of Reactions: 100 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Detection Method: Gel Electrophoresis
• GC-Rich PCR Performance: High
• Polymerase: Platinum SuperFi
• Technique: 1-step RT-PCR
• Reverse Transcriptase: SuperScript IV
• Shipping Condition: Dry Ice
• Hot Start: Built-In Hot Start
• Reaction Format: Master Mix
• Size (Final Product): Up to 14 kb
• Includes: Kit with ezDNase

For Research Use Only. Not for use in diagnostic procedures.