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MilliporeSigma™ Stem Cell Qualified ECM Gel
Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.
Supplier: MilliporeSigma™ CC1315ML10P
Description
- Stem Cell Qualified ECM Gel is a soluble basement membrane extract (BME) purified from Engelbreth-Holm-Swarm (EHS) tumor that has been pre-qualified for human ES/iPS cell culture
- The matrix polymerizes at 20 to 40°C to form a reconstituted basement membrane
- ECM Gel contains laminin, collagen IV, entactin and heparan sulfate among other ECMs
- The matrix has been extensively tested and optimized for human ES/iPS cell culture
- Eliminates the need for time-consuming prescreening and lot identification
- Provides an optimized ECM coating necessary for long term culture of pluripotent human ES/iPS cells
- Supports feeder-free culture of human ES/iPS cells in serum-free media
- Compatible with all feeder free human ES/iPS cell expansion media (i.e. mTeSR™, PluriSTEM™)
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Specifications
< 5% spontaneous differentiation for 3 passages using Human iPS cells | |
Store at -20°C, thaw overnight on ice in a 2 to 8°C refrigerator | |
Gel | |
Human ES and iPS cell culture |
Red, clear of particulates | |
Basement membrane extract (BME) | |
< 20 EU/mL | |
10 x 5 mL |
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