MilliporeSigma™ Stem Cell Qualified ECM Gel

Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.

Supplier:  MilliporeSigma™ CC1315ML

Catalog No. CC1315ML

Description

• Stem Cell Qualified ECM Gel is a soluble basement membrane extract (BME) purified from Engelbreth-Holm-Swarm (EHS) tumor that has been pre-qualified for human ES/iPS cell culture
• The matrix polymerizes at 20 to 40°C to form a reconstituted basement membrane
• ECM Gel contains laminin, collagen IV, entactin and heparan sulfate among other ECMs
• The matrix has been extensively tested and optimized for human ES/iPS cell culture
• Eliminates the need for time-consuming prescreening and lot identification
• Provides an optimized ECM coating necessary for long term culture of pluripotent human ES/iPS cells
• Supports feeder-free culture of human ES/iPS cells in serum-free media
• Compatible with all feeder free human ES/iPS cell expansion media (i.e. mTeSR™, PluriSTEM™)

Specifications

• Assays: < 5% spontaneous differentiation for 3 passages using Human iPS cells
• Content And Storage: Store at -20°C, thaw overnight on ice in a 2 to 8°C refrigerator
• Form: Gel
• For Use With (Application): Human ES and iPS cell culture

• Color: Red, clear of particulates
• Description: Basement membrane extract (BME)
• Endotoxin Level: < 20 EU/mL
• Quantity: 5 mL