Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO

Important Note: These G2 Dialysis Cassettes will be discontinued on June 1, 2023 and replaced by G3 Dialysis Cassettes, a new generation of cassettes. Consider switching to G3 Dialysis Cassettes. Remove salts and contaminants while maximizing sample recovery with these second-generation, self-floating protein dialysis cassettes.

Catalog Number	Mfr. No.	Description	Sample Volume (Metric)
PI87729	Thermo Scientific™ 87729	G2 Dialysis Cassettes	0.5 mL
PI87730	Thermo Scientific™ 87730	G2 Dialysis Cassettes	3 mL
PI87731	Thermo Scientific™ 87731	G2 Dialysis Cassettes	15 mL
PI87732	Thermo Scientific™ 87732	G2 Dialysis Cassettes	30 mL
PI87733	Thermo Scientific™ 87733	G2 Dialysis Cassettes	70 mL
PI88250	Thermo Scientific™ 88250	G2 Dialysis Cassettes (gamma-irradiated)	0.5 mL
PI88251	Thermo Scientific™ 88251	G2 Dialysis Cassettes (gamma-irradiated)	3 mL
PI88252	Thermo Scientific™ 88252	G2 Dialysis Cassettes (gamma-irradiated)	15 mL
PI88253	Thermo Scientific™ 88253	G2 Dialysis Cassettes (gamma-irradiated)	30 mL
PI88254	Thermo Scientific™ 88254	G2 Dialysis Cassettes (gamma-irradiated)	70 mL

Description

Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility, and performance for sample dialysis. They allow samples to be loaded with a syringe or pipette through the unique sample port. The sample is easily recovered through either the pipette or syringe sample ports. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems.

Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or pipette through the unique sample port. The cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules larger than 10,000 Daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
• Multiple sizes—five cassette capacities to optimally match 0.5 to 70 mL sample volumes
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

Gamma-irradiated (sterile) dialysis cassettes
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA, and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20–36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Membrane specifications:
• Composition: regenerated cellulose synthesized by the viscose method
• Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
• Hydration time required before use: 2 minutes
• Glycerol content: 21%
• Sulfur content: 0.1–0.15%
• Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.25 to 0.75 mL
• 3 mL = 1 to 3 mL
• 15 mL = 5 to 15 mL
• 30 mL = 10 to 30 mL
• 70 mL = 25 to 70 mL

Specifications

• Content And Storage: Store at room temperature
• Membrane: Cellulose Membrane, Dialysis
• MWCO: 10 kDa

• Purification Target: Buffer Exchange, Protein
• Format: Cassette
• Product Line: Slide-A-Lyzer™