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Cytiva RNAspin 96 RNA Isolation Kit

For fast and efficient purification in a 96-well format

Supplier:  Cytiva 25050075

Catalog No. 45-001-757


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Includes: Protocol and sufficient quantities of the following to carry out the number of preps indicated: DNase 1, RNase-free water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and buffers

Description

Description

  • 96 samples parallel-processed via either centrifugation or vacuum with high reproducibility in under 70 minutes
  • Can be used to isolate total RNA from cultured cells, tissue, bacteria or yeast
  • DNase I treatment removes genomic DNA resulting in high-quality total RNA product
  • Isolated RNA is of sufficient quality (A260/A280 = 1.8-2.2) and quantity (up to 100μg) for multiple downstream experiments: quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension and RNase protection assays
  • Integrated wash plate eliminates risk of contamination
  • Accessory tissue plate allows tissue samples to be processed
  • Sample size: up to 30mg for animal tissue, up to 2 x 106 cells for cell culture (vaccum); 1x 107 cells for cell culture (centrifuge); Elution volume: 50μ-130μL

Specifications

Specifications

illustra RNAspin 96 Kit
Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeastCan also be used in a fully automated flow with vacuum on common laboratory workstationsSuitable for direct use in downstream application
4 x 96 Preps
1.8-2.2
70 min/4 preps
96-well Plate
Protocol and sufficient quantities of the following to carry out the number of preps indicated, DNase 1, RNase-free Water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and Buffers
illustra RNAspin 96 Kit
10 mg tissue-30 mg tissue
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