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Thermo Scientific Chemicals RIPA buffer (5X)

RIPA buffer, radio immunoprecipitation assay buffer, is used for whole cell isolation of proteins from tissues and cell culture.

Specifications

Content And Storage 2° to 8°C
Format Liquid
Product Type Cell Lysis Reagent
Concentration 5X
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
AAJ62524AD
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Thermo Scientific Chemicals
J62524AD
50mL
Each for $121.90
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N/A
AAJ62524AE
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Thermo Scientific Chemicals
J62524AE
100mL
Each for $209.50
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Request bulk or custom formats

N/A
Edge
Description

Description

RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• RIPA can be used for isolation of cytoplasmic, membrane, nuclear, and mitochondrial proteins
• Protease inhibitors can be added to RIPA buffer to assist in protein isolation

Application

• RIPA buffer is suitable for use in protein isolation from culture cells or homogenized tissues
• Downstream applications of RIPA buffer include ELISA, western blot, immunoprecipitation, and SDS-PAGE

Specifications

Specifications

2° to 8°C
Liquid
Cell Lysis Reagent
5X
Safety and Handling

Safety and Handling

Recommended Storage : Keep cold

SDS
Documents

Documents

Product Certifications

RUO – Research Use Only

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