Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Supplier:  Thermo Scientific™ 46430

Catalog No. PI46430

Description

Thermo Scientific™ Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.

Highlights:

• Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature

• Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate

• Cost effective – save valuable time and samples; strip blots effectively the first time

• Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times

• Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies

Specifications

• Product Type: Western Blot Stripping Buffer
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 500 mL

• Detection Method: Chemiluminescent
• For Use With (Application): Western Blot
• Product Line: Restore™
• Content And Storage: Upon receipt store at room temperature. Product shipped at ambient temperature.

For Research Use Only. Not for use in diagnostic procedures.