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Thermo Scientific™ TCBS Agar
Selectively isolate Vibrio cholerae and other enteropathogenic Vibrio spp. from clinical and non-clinical specimens using TCBS Agar.
$136.00 - $403.00
Specifications
| Certifications/Compliance | Industrial Reference: AOAC, BAM, COMPF. |
|---|---|
| Description | Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar |
| Product Type | Agar |
Description
Selectively isolate Vibrio cholerae and other enteropathogenic Vibrio spp. from clinical and non-clinical specimens using Thermo Scientific™ Remel™ TCBS Agar (Thiosulfate, Citrate, Bile Salts, Sucrose). In 1963, Kobayashi1 et al. modified Thiosulfate-Citrate-Bile Salts-Sucrose Agar (TCBS) developed by Nakanishi2. It was developed for selective isolation of vibrios which cause cholera, diarrhea, and food poisoning. In 1982, West et al. reported that TCBS Agar could be used for recovery of certain new pathogens such as Vibrio fluvialis and Vibrio vulnificus3.
TCBS Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio spp. Vibrio cholerae is a gram-negative bacterium that causes cholera outbreaks and epidemics. Other Vibrio species such as V. parahaemolyticus, V. vulnificus, V. mimicus are well known human pathogens. TCBS Agar has a high pH that enhances the recovery of V. cholerae and supresses the growth of other intestinal microorganisms. Coliforms, Proteus, Pseudomonas, and Aeromonas are partially inhibited in this medium. Based on their ability to ferment sucrose, Vibrio spp. produce yellow or green colonies in this medium.
- Recommended by the World Health Organization (WHO) for isolation of Vibrio cholerae4
- Recommended by AOAC International (AOAC)5
- Selective medium – Oxgall contains a mixture of bile salts which inhibits gram-positive organisms
- Ready-to-use – Convenience of prepared media
The medium contains casein and meat peptones that supply nutrients necessary for bacterial growth. Yeast extract is a growth enhancer. Oxgall inhibits gram-positive organisms. Sodium thiosulfate along with ferric citrate detects the production of hydrogen sulfide. Sucrose serves as a source of carbohydrate which is fermented by Vibrio spp. The pH indicator system is made up by brom thymol blue and thymol blue.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
| Industrial Reference: AOAC, BAM, COMPF. | |
| Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar | |
| Agar |
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