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Thermo Scientific™ MacConkey Broth

Selectively and differentially isolate enteric gram-negative bacilli using MacConkey Broth.

Supplier:  Thermo Scientific™ R061336

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Catalog No. R061336


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Description

Description

Selectively and differentially isolate enteric gram-negative bacilli using Thermo Scientific™ Remel™ MacConkey Broth. MacConkey Broth is a modification of the original bile salt broth first described by MacConkey in 1900, which contained litmus as an indicator and sodium taurocholate to inhibit gram-positive organisms1. In further testing, MacConkey recommended replacing litmus with neutral red2. In 1953, Childs and Allen replaced neutral red with bromcresol purple, a less inhibitory indicator3.

Selectively and differentially isolate enteric gram-negative bacilli using MacConkey Broth. This medium is used for presumptive identification of coliforms. Coliforms are organisms that are present in the environment and in the feces of all warm-blooded animals and humans.

Their presence in drinking water indicates that disease-causing organisms (pathogens) could be in the water system.

  • Selective: Oxgall is a selective agent which inhibits gram-positive organisms.
  • Ease of differentiation: Lactose and bromcresol purple indicator enable the differentiation of lactose fermenting gram-negative bacilli.
  • Conforms to USP and EP: It is formulated and tested in conformance with harmonized United States Pharmacopeia (USP)/European Pharmacopeia (EP) guidelines4.

This medium contains gelatin peptone that provides amino acids and essential nutrients for bacterial growth. Lactose is a carbon source for energy. Oxgall is a selective agent which inhibits gram-positive organisms. Lactose and bromcresol purple indicator enable the differentiation of lactose fermenting gram-negative bacilli.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Specifications

USP
D - 5mL
20 x 5 mL
MacConkey Broth
Microbiology Media
SDS
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