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Rat IgG Isotype Control, Invitrogen™
Rat Isotype Control
Supplier: Invitrogen 10700
Description
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Rat IgG is a type of immunoglobulin G (IgG) antibody that is commonly used in scientific research and diagnostics. It is produced by rats in response to foreign antigens, and is widely used as a tool for detecting and measuring the presence of specific proteins or other molecules in biological samples.Specifications
Rat IgG | |
3 mg/mL | |
PBS with 0.1% sodium azide | |
Rat | |
IgG fraction | |
RUO | |
Not Applicable | |
Liquid |
Control | |
Unconjugated | |
Immunoglobulin G; ImmunoglobulinG | |
Gamma Immunoglobin | |
5 mL | |
Isotype Control (Primary) | |
4° C | |
IgG |
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