Rat anti-Mouse IgG1 Secondary Antibody, APC, eBioscience™, Invitrogen™

Rat Monoclonal Secondary Antibody

Supplier:  Invitrogen 17401580

Catalog No. 17-401-580

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG1 Monoclonal antibody specifically detects Mouse IgG1 in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen).

Specifications

• Antigen: Mouse IgG1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: M1-14D12
• Conjugate: APC
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG

For Research Use Only.