Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to protein standard curve of known concentrations.

Catalog Number	Mfr. No.	Quantity
PIA53227	Thermo Scientific™ A53227	20 mL
PIA53226	Thermo Scientific™ A53226	250 mL
PIA53225	Thermo Scientific™ A53225	500 mL

Description

• A fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration
• Can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications
• Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods
• Provides comparable accuracy but with a 5 min., room temperature incubation and measured at 480 nm
• This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results
• Exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Linear working range for BSA of 20 to 2000 μg/mL
• Store in refrigerator at 2°C to 8°C

Specifications

• Assay Range: 20 to 2000 μg/mL
• Assay: Rapid Gold BCA Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Equipment): Spectrophotometer, Microplate Reader

• Content And Storage: Store in refrigerator (2–8°C).
• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• For Use With (Application): Solution-based Detection, Absorbance
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.