Rabbit anti-DDDDK Tag (FLAG Tag), MP Biomedicals™

Rabbit Monoclonal Antibody

Supplier:  MP Biomedicals, Inc 08L100031

Catalog No. MP08L100031

Description

• Rabbit anti-DDDDK Tag (FLAG Tag) mAb offers high specificity and potency and consistency against FLAG Tag, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), and Immunofluorescence (IF) assays.
• FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
• It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism.
• It can also be used in the isolation of protein complexes with multiple subunits because its mild purification procedure tends not to disrupt such complexes.
• It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.
• A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG tag to a protein allows the protein to be studied with an antibody against the FLAG sequence.

Specifications

• Antigen: DDDDK Tag (FLAG Tag)
• Classification: Monoclonal
• Formulation: In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol; pH 7.3 to 7.4
• Immunogen: Synthetic peptide corresponding to DDDDK tag
• Quantity: 100 μL
• Primary or Secondary: Secondary
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Rabbit
• Purification Method: Affinity Purification
• Regulatory Status: RUO
• Form: Purified

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

Unless specified otherwise, for research or further manufacturing use only, not for direct human use.