Abnova™ PTEN/CEN10 CISH Probe

PTEN/CEN10 CISH Probe is designed for the qualitative detection of human PTEN gene and chromosome 10 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0012

Catalog No. 89-335-553

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dots (PTEN gene region), and dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 10).
Normal situation: In interphases of normal cells or cells without a deletion involving the PTEN gene, two green signals and two red signals appear.
Aberrant situation: In a cell with deletions of the PTEN gene locus, a reduced number of green signals will be observed. Deletions affecting only parts of the PTEN gene might result in normal signal pattern with green signals of reduced size. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P