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Cytiva Protein A HP SpinTrap™ Column

For simple, small-scale antibody purification or enrichment of specific proteins by antibody capture in single-use spin columns

Supplier:  Cytiva 28903132

Catalog No. 45-001-483

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Prepacked with Protein A Sepharose™ High Performance Media for coupling of antibodies of IgG subclasses; used with a standard microcentrifuge

  • Fast binding kinetics and high capacity provide high yield
  • Classic (antigen eluted with antibody) and cross-link (antigen eluted separately) protocols provide flexibility
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows
  • Easy scale-up with HiTrap Protein A HP prepacked columns
  • Additional protocol for small-scale purificaiton of monoclonal and polyclonal antibodies from serum and cell culture supernatants



>1 mg Human IgG well
600 μl
2°C to 30°C, 20% Ethanol
Standard microcentrifuge.
16 Columns




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