Cytiva Prostaglandin E2 Biotrak™ Assay

Enzyme immunoassay system suitable for plasma, urine, or cell culture supernatant samples

Supplier:  Cytiva RPN222

Catalog No. 45-000-887

Includes: RPN222 supplied with sufficient quantities of reagents for 96 wells; RPN22210, for 960 wells. RPN22210 does not include lysis reagents. Both allow for construction of one standard curve and measurement of 37 unknowns in duplicate per plate.

Description

• Specific for PGE₂
• Flexible method with choice of five assay protocols
• Product available with or without lysis reagents
• Lysis reagents facilitate extraction of PGEsubscript 2 from cell cultures and plasma samples; PGE is directly available for subsequent analysis without removal of extraction reagents
• Precise and accurate measurement
• Ready to use substrate and color-coded reagents
• Range: 20-640pg/mL (1-32pg/well)
• Room temperature protocol: 50-6400pg/mL
• Sensitivity: 16pg/mL, 0.8pg/well
• 5-hour protocol
• Storage: Store at -15° to -30°C.

Specifications

• Assay Range: 20 to 640pg/mL
• Quantity: 96 Wells
• Sample Volume: 50 μL
• Total Assay Time: 5 hr.
• For Use With (Application): ELISA

• Includes: Microplate (12 x 8-well strip coated with sheep Anti-Mouse IgG), Assay Buffer, standard (prostaglandin E2) 256ng/mL, prostaglandin E Antibody (lyophilized), Prostaglandin E2 Conjugate, Wash Buffer, TMB Substrate, lysis reagent 1, lysis reagent 2
• Sample Type: Plasma (EDTA, Citrate), Urine, Tissue Homogenates, Cell Culture, Cell Culture Supernatant
• Sensitivity: 16 pg/mL (0.8 pg/Well)
• Content And Storage: -15°C to -30°C
• Product Type: Prostaglandin E2 Direct Biotrak assay