Promega TNT™ Quick Coupled Transcription/Translation System

The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.

Catalog Number	Mfr. No.	Includes	No. of Reactions
PR-L1170	Promega L1170	8 × 200μL TNT Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 250μL Luciferase Assay Reagent, 50μL Methionine, 1250μL Nuclease-free Water	40
PR-L1171	Promega L1171	200μL TNT T7 Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 50μL Methionine	5
PR-L2080	Promega L2080	8 × 200μL TNT SP6 Quick Master Mix, 5μg Luciferase SP6 Control DNA, 50μL Methionine, 250μL Luciferase Assay Reagent, 1250 Nuclease-free Water	40
PR-L2081	Promega L2081	200μL TNT SP6 Quick Master Mix, 5μg Luciferase SP6 Control DNA, 50μL Methionine	5

Description

• Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression
• Functional protein is expressed in one hour, ready for use without further purification
• Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
• Choice of SP6 or T7 promoter

U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents.
U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329 and other patents.
U.S. Patent 5,552,302; Australian Patent 646803 and other patents.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
For Laboratory Use. Any use of the product for diagnostics requiring clearance or approval by the FDA may require a license under Mayo Clinic U.S. Patents 6,027,913 and 6,361,949.

Specifications

• Content And Storage: <-65°C
• For Use With (Application): GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)