Thermolysin is a thermostable metalloproteinase. The high digestion temperatures may be used as an alternative to denaturants to improve digestion of proteolytically resistant proteins. The optimal digestion temperature range is 65-85°C.
Supplier: Promega V4001
- Thermostable Proteinase with High Digest Temperature; Better Denaturation, Digestion of Proteolytically Resistant Proteins
- Cleaves at the N-terminus of Leu, Phe, Val, Ile, Ala, Met
- Optimal digestion temperature is 65-85°C
- Optimal activity at pH 5.0-8.5
|−30 to −10°C|
|149°F to 149°F|
|65°C to 85°C|
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