Promega E. coli T7 S30 Extract System for Circular DNA
The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.
Supplier: Promega L1130
- Create Radiolabeled Protein for Transcription/Translation Studies
- Translate using clones with a T7 promoter and a ribosome binding site; no E. coli promoter required
- Reduce the chance of expressed proteins degrading
- Includes all components needed for coupled transcription/translation
|Synthesis of radiolabeled Protein, verification of cloned gene expression before in-vivo Protein expression in E. coli, use of Protein in functional studies of transcription and translation, use of Protein as a tracer in Protein purification, incorporation of un-natural Amino Acids into Proteins for structural studies|
|5μg PinPoint Xa control vector, 3 x 150μL T7/S30 extract for circular DNA, 750μL S30 premix without Amino Acids, 175μL Amino Acid mixture minus Methionine, 175μL Ami|
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