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Promega E. coli T7 S30 Extract System for Circular DNA

The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.

Supplier:  Promega L1130

Catalog No. PR-L1130

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Includes: PinPoint™ Xa Control Vector, 5μg; T7/S30 extract for circular DNA, 3 × 150μL; S30 premix without amino acids, 750μL; Amino acid mixture minus methionine, 175μL; Amino acid mixture minus cysteine, 175μL; Amino acid mixture minus leucine, 175μL



  • Create Radiolabeled Protein for Transcription/Translation Studies
  • Translate using clones with a T7 promoter and a ribosome binding site; no E. coli promoter required
  • Reduce the chance of expressed proteins degrading
  • Includes all components needed for coupled transcription/translation


Synthesis of radiolabeled Protein, verification of cloned gene expression before in-vivo Protein expression in E. coli, use of Protein in functional studies of transcription and translation, use of Protein as a tracer in Protein purification, incorporation of un-natural Amino Acids into Proteins for structural studies
5μg PinPoint Xa control vector, 3 x 150μL T7/S30 extract for circular DNA, 750μL S30 premix without Amino Acids, 175μL Amino Acid mixture minus Methionine, 175μL Ami


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