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PLC/PRF/5 (human hepatoma) Whole cell lysate, Non-denatured; Abnova

Supplier:  Abnova Corporation L011V2

Catalog No. 89-014-369

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Western Blotting, Immunoprecipitation



Whole cell lysate (non-denatured)
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
200 μg
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Immunoprecipitation, Western Blot
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
12.5% SDS-PAGE Stained with Coomassie Blue.
In modified RIPA Lysis Buffer.
5 mg/mL
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