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Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Manufacturer:  Thermo Scientific™ N600

Catalog No. PIN600

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Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5« - tetramethylbenzidine (TMB).

TMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5«-tetramethylbenzidine (TMB). Addition of sulfuric acid stop solution changes the color from blue to yellow, stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.
  • Size: 55mL

  • Sufficient For: five 96-well microplates

  • Application: ELISA

Instructions: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 to 100μL per well). Use at full strength.

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Store at 2–8°C.
Stop Solution
55 mL
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