Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Supplier:  Thermo Scientific™ 24612

Catalog No. PI24612

Description

Thermo Scientific™ Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types for both first-time and experienced users.

The Pierce Silver Stain Kit is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Highlights:

• Sensitive silver stain – detect proteins at 0.25ng; equal or better sensitivity than monochromatic metallic silver stains from other suppliers

• Clear background – method produces intensely-stained protein gels with very low background color, shading or anomalies

• Robust performance – reagents and protocol are formulated to yield excellent staining results amidst even wide-ranging differences in user technique

• Rapid, flexible protocol – approx. 1.5 hours are required to complete the entire default procedure, but certain steps can be performed overnight or shortened with only minimal effects

• Gel-compatible – staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems

• Workflow-compatible – mild chemical formulation ensures compatibility with mass spectrometry, sequencing and other downstream procedures that depend on destaining and protein recovery

Additional Features:

• Gel fixation step can be completed in 30 minutes or overnight
• Gel staining can be performed within 5 min to 20 hrs, depending on required sensitivity

Includes:

Sensitizer, stain, developer and enhancer solutions (1.03L total)

Specifications

• Content And Storage: Sufficient For: 20 mini gels (SDS-PAGE)
  • Silver Stain Sensitizer, 2 mL
  • Silver Stain, 500 mL
  • Silver Stain Enhancer, 25 mL
  • Silver Stain Developer, 500 mL
  
  Store at room temperature.
• Detection Method: Colorimetric
• Product Type Specs: Stain Kit
• Target Molecule: Protein

• Detection Location: In-Gel Detection
• Label or Dye: Silver
• Product Line: Pierce™