Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Saturated Ammonium Sulfate Solution

Fractionate immunoglobulin, antibody, or other proteins by selective precipitation, also called salting out.

Supplier:  Thermo Scientific™ 45216

Catalog No. PIA45216


Explore available promotions
Add to Cart

Description

Description

Thermo Scientific™ Pierce Saturated Ammonium Sulfate Solution is formulated and ready to use for simple immunoglobulin, antibody, or other protein fractionation applications based on selective precipitation, which is also called salting out.

The saturated ammonium sulfate solution (4.32M) enables removal of abundant proteins from serum, cell culture supernatant and ascites fluid. Slowly adding the solution to a protein sample results in decreased protein solubility and eventual protein precipitation as the charged ammonium sulfate ions compete with protein for water interaction. High molecular weight proteins, such as antibodies, are among the first to precipitate. When used in combination with antibody purification methods such as affinity chromatography, ammonium sulfate precipitation is ideal for achieving highly purified antibody samples.

Highlights:

  • Convenient – solution is ready to use; no preparation required

  • Concentrates – recover antibody in 100mL from more than 1L of cell culture supernatant

  • Improves purity – partial-purification of antibodies by ammonium sulfate precipitation greatly improves the final purity that can be obtained with affinity purification

  • Extends column life – using pre-purified antibody samples instead of crude samples enables affinity columns to be used for many more cycles

  • Compatible – ammonium sulfate is easily removed by dialysis or desalting columns, eliminating any obstacles to downstream methods

  • Versatile – use for precipitating immunoglobulins or “salting out” fractions of other sample types

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Ammonium Sulfate Solution
Affinity
Upon receipt store at ambient temperature.
Liquid
Pierce™
Precipitation Reagent
1 L
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

For Research Use Only. Not for use in diagnostic procedures.