Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Recombinant Protein A Agarose
GSA_VA

Purify IgG using this genetically engineered, immobilized protein A on beaded agarose, when native protein A has undesirable effects.

$514.00 - $1808.00

Specifications

Content And Storage Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Column Type Agarose Resin, Affinity
Format Bottle
Product Line Pierce™
Product Type Agarose
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PI20365
View Documents
Thermo Scientific™
20365
5 mL
Pack for $514.00
Add to cart
 
PI20366
GSA_VA
View Documents
Thermo Scientific™
20366
25 mL
Each for $1,808.00
Add to cart
 
Description

Description

Thermo Scientific™ Pierce Recombinant Protein A Agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilized for use in IgG purification applications where the native ligand has undesirable effects.

The Pierce Recombinant Protein A Agarose uses a genetically engineered form of Protein A that is produced in E. coli and then immobilized on crosslinked 6% beaded agarose (CL-6B). Although some nonessential regions have been removed, recombinant is similar to native Protein A in overall size and IgG-binding domains. The protein has four high affinity binding sites capable of specifically binding to the Fc region of immunoglobulin molecules from several species. Some researchers prefer to use an affinity resin based on the recombinant form of Protein A in particular applications where practical experience indicates that the different production source (E. coli vs. S. aureus) provides less nonspecific binding or lower levels of endotoxin contamination. Otherwise, native Protein A resins generally provide higher capacity antibody purification.

Highlights:

  • Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
  • Standard capacity – loading of immobilized Protein A provides a binding capacity of 15 to 17mg human IgG per milliliter of resin
  • Agarose resin – crosslinked 6% beaded agarose for small column or batch affinity purification methods

Properties of crosslinked 6% beaded agarose (CL-6B):

  • Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
  • Average Particle Size: 45 to 165 microns
  • Exclusion Limit: 10,000 to 4,000,000 daltons
  • Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
  • Maximum Linear Velocity: 30cm per hour
  • Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)
Specifications

Specifications

Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Bottle
Agarose
IgG Antibodies
Agarose Resin, Affinity
Pierce™
Protein A
SDS
Product Certifications
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit