Thermo Scientific™ Pierce™ Protein Coated Plates

Thermo Scientific™ Pierce Protein A, G, A/G and L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Catalog Number	Mfr. No.	Surface Treatment	Description
PI15132	Thermo Scientific™ 15132	Protein A	Clear, 8-well Strip
PI15130	Thermo Scientific™ 15130	Protein A	Clear, 96-well
PI15155	Thermo Scientific™ 15155	Protein A	Black, 96-well
PI15154	Thermo Scientific™ 15154	Protein A	White, 96-well
PI15133	Thermo Scientific™ 15133	Protein G	Clear, 8-well Strip
PI15131	Thermo Scientific™ 15131	Protein G	Clear, 96-well
PI15157	Thermo Scientific™ 15157	Protein G	Black, 96-well
PI15156	Thermo Scientific™ 15156	Protein G	White, 96-well
PI15138	Thermo Scientific™ 15138	Protein A/G	Clear, 8-well Strip
PI15190	Thermo Scientific™ 15190	Protein L	Clear, 96-well

Description

These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Highlights
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Plates
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Protein A Coated Plates
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Protein G Coated Plates
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Protein L Coated Plates
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Specifications

• Binding Property: Standard Capacity, Affinity
• Material: Polystyrene
• No. per Pack: 5 Plates

• Product Line: Pierce™
• No. of Wells: 96
• Plate Blocking: SuperBlock™ Buffer