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Thermo Scientific™ Pierce™ Protein Coated Plates

Thermo Scientific™ Pierce Protein A, G, A/G and L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

$302.00 - $893.00

Specifications

Binding Property Standard Capacity, Affinity
Product Line Pierce™
Material Polystyrene
No. of Wells 96
No. per Pack 5 Plates
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Products 10
Catalog Number Mfr. No. Surface Treatment Description Price Quantity & Availability  
Catalog Number Mfr. No. Surface Treatment Description Price Quantity & Availability  
PI15132
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Thermo Scientific™
15132
Protein A Clear, 8-well Strip
Pack of 5 for $303.00
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PI15130
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Thermo Scientific™
15130
Protein A Clear, 96-well
Pack of 5 for $893.00
Add to cart
 
PI15155
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Thermo Scientific™
15155
Protein A Black, 96-well
Pack of 5 for $305.00
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PI15154
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Thermo Scientific™
15154
Protein A White, 96-well
Pack of 5 for $302.00
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PI15133
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Thermo Scientific™
15133
Protein G Clear, 8-well Strip
Pack of 5 for $329.00
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PI15131
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Thermo Scientific™
15131
Protein G Clear, 96-well
Pack of 5 for $307.00
Add to cart
 
PI15157
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Thermo Scientific™
15157
Protein G Black, 96-well
Pack of 5 for $330.00
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PI15156
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Thermo Scientific™
15156
Protein G White, 96-well
Pack of 5 for $332.00
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PI15138
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Thermo Scientific™
15138
Protein A/G Clear, 8-well Strip
Pack of 5 for $331.00
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PI15190
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Thermo Scientific™
15190
Protein L Clear, 96-well
Pack of 5 for $323.00
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Description

Description

These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Highlights
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Plates
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Protein A Coated Plates
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Protein G Coated Plates
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Protein L Coated Plates
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Specifications

Specifications

Standard Capacity, Affinity
Polystyrene
5 Plates
Pierce™
96
SuperBlock™ Buffer
SDS
Product Certifications
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