Thermo Scientific™ Peroxidase Suppressor
Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.
Supplier: Thermo Scientific™ 35000
Thermo Scientific™ Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.
The immunoperoxidase method is a commonly used detection method for immunostaining of cells and tissues. However, the analysis often is complicated by the presence of endogenous peroxidase and “pseudoperoxidase” activity in the cells and tissues. This presence results in nonspecific staining when a substrate such as diaminobenzidine (DAB) is present. Nonspecific staining is difficult to distinguish from the immunochemical marker, making it difficult to distinguish the final location of the antigen of interest. The typical method of inhibiting endogenous peroxidase activity involves hydrogen peroxide or hydrogen peroxide in methanol. The peroxidase suppressor inhibits peroxidase activity even more effectively than the method using hydrogen peroxide in methanol. Plus, the Peroxidase Suppressor does not affect antibody activity. Peroxidase Suppressor also can be used on blots of whole cell supernatant prior to the addition of the horseradish peroxidase (HRP) marker.
Effectively inhibits peroxidase activity without interfering with antigen-antibody interactions
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For Research Use Only. Not for use in diagnostic procedures.