Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific™ 36000

Catalog No. PI36000

Description

Thermo Scientific™ Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The Thermo Scientific Pierce Metal Enhanced DAB Substrate Kit optimizes this intensifying chemistry, producing an exceptionally sensitive colorimetric detection system for Western blot, dot-blot and immunohistochemistry applications. The near-black signal (precipitate) is easy to photograph.

Highlights:

• Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

• Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

• Six-hour stability – working solution is stable for more than six hours at room temperature! Other DAB substrates must be used immediately

• Convenient – eliminates the need to procure numerous reagents from various sources

Includes:

DAB/Metal Concentrate (10X), 25mL; Universal Blocker Blocking Buffer in TBS, 250mL; Peroxidase Suppressor, 2 × 100mL; Stable Peroxide Buffer, 250mL; BupH Tris Buffered Saline, 4 packs; Surfact-Amps 20 (10% Tween-20), 10mL; Harris Modified Hematoxylin, 100mL; Mounting Medium, 60mL

Specifications

• Detection Method: Chromogenic, Colorimetric
• Substrate: DAB (Enhanced)
• Form: Liquid
• For Use With (Application): Immunodetection, IHC (Immunohistochemistry)

• Content And Storage:
  • DAB/Metal Concentrate (10X), 25 mL, store at -20°C (this solution will not freeze at -20°C)
  • Universal Blocker Blocking Buffer in TBS, 250 mL, store at 4°C
  • Peroxidase Suppressor, 2 × 100 mL, store at 4°C
  • Stable Peroxide Buffer, 250 mL, store at 4°C
  • BupH Tris Buffered Saline, 4 packs (each pack makes 500 mL), store at room temperature
  • Surfact-Amps 2 (1% Tween-2 Detergent), 10 mL, store at 4°C
  • Harris Modified Hematoxylin (without acetic acid, mercury-free), 100 mL, store at room temperature
  • Mounting Medium, 120 mL, store at room temperature
• Substrate Type: HRP (Horseradish Peroxidase) Substrate
• Product Line: Pierce™
• Product Type: Peroxidase IHC Detection Kit

For Research Use Only. Not for use in diagnostic procedures.