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Thermo Scientific™ Pierce™ Nickel Coated Plates

Thermo Scientific™ Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.

$316.00 - $323.00

Specifications

Binding Property Standard Capacity, Affinity
Content And Storage Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
Packaging 5 Plates
Product Line Pierce™
Product Type Microplate
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Products 4
Catalog Number Mfr. No. Description Detection Method Price Quantity & Availability  
Catalog Number Mfr. No. Description Detection Method Price Quantity & Availability  
PI15342
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Thermo Scientific™
15342
Black, 96-well Fluorescence
Pack of 5 for $323.00
Add to cart
 
PI15142
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Thermo Scientific™
15142
Clear, 8-well Strip Colorimetric
Pack of 5 for $316.00
Add to cart
 
PI15442
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Thermo Scientific™
15442
Clear, 96-well Colorimetric
Pack of 5 for $323.00
Add to cart
 
PI15242
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Thermo Scientific™
15242
White, 96-well Chemiluminescence
Pack of 5 for $323.00
Add to cart
 
Description

Description

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.

Features of Pierce Nickel Coated Plates include:
• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1 ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Specifications

Specifications

Standard Capacity, Affinity
5 Plates
Microplate
200 μL
Divalent Nickel, Metal Chelate
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
Pierce™
Polystyrene
96
Blocker™ BSA Buffer
SDS
Product Certifications
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