Thermo Scientific™ Pierce™ NeutrAvidin™ Coated High Capacity Plates
Thermo Scientific™ Pierce NeutrAvidin High Binding Capacity (HBC) Coated Plates are pre-blocked and ready-to-use polystyrene microplates for assays involving biotinylated antibodies or biotin-labeled molecules.
Supplier: Thermo Scientific™ 15511
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Thermo Scientific NeutrAvidin Protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding. With a near-neutral pI (6.3), this results in lower nonspecific interactions in certain applications compared with streptavidin. NeutrAvidin Protein lacks the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells.
Thermo Scientific NeutrAvidin™ Coated High Capacity Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.
Features of Thermo Scientific Pierce Coated Plates include:
Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Robust and sensitive—NeutrAvidin Protein yields less nonspecific binding for improved signal-to-noise ratios
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—choose from 96- and 384-well formats in a variety of colors
The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.
For detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins, check out
Pierce™ NeutrAvidin™ Coated High Sensitivity Plates, Clear, 8-Well Strip
|High Capacity, Affinity|
|Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.|
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