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Thermo Scientific™ Pierce™ Microdialysis Plates
Simultaneously process 1 to 96 samples with 10 to 100 μL volumes with this convenient rapid dialysis system that is automation compatible and disposable.
Supplier: Thermo Scientific™ A50467
Description
Pierce 48-well and 96-well, 2K, 3.5K, 10K, and 20K MWCO microdialysis plates are an automation-compatible microplate with devices for the rapid dialysis of 1 to 96 samples with volumes of 10 to 1000 μL. Using the Pierce Microdialysis Plate, sample dialysis can be efficiently performed in a standard 96 deep-well plate with a minimal amount of buffer while still providing >95% removal of small molecules. The plate is compatible with standard 96-well laboratory equipment and automated liquid-handling systems, making it an ideal option for high-throughput applications.
Each sample processed with the Pierce Microdialysis Plate is dialyzed inside a single, removable dialysis device consisting of two regenerated cellulose membranes ∼2 mm apart. The short diffusion distance and large surface area facilitate rapid dialysis, with the removal of salts and small molecules often complete in 2–4 hours. Sample is recovered using standard laboratory micropipettes. The plate seals are made of high-quality polypropylene and help protect the samples from contamination and evaporation, especially during long-term storage.Features of Pierce Microdialysis Plate:
• High yield—greater than 90% protein recovery
• Rapid—dialysis completed in 2–4 hours
• Easy to use—sample loading and retrieval can be done with a standard pipette tip
• Flexible—detachable 8-device strips, scalable from 1 to 96 samples
• Automation-compatible—plate format conforms to the SBS Microplate Standard
Each 48-well and 96-well Pierce Microdialysis Plate comes pre-loaded with twelve 8-dialysis device strips. Each dialysis device strip can be removed from the plate and separated into individual microdialysis devices, allowing only the number of needed devices to be used and eliminating waste. The sample chamber inside each microdialysis device is formed by two low-binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules >2,000 Daltons while allowing removal of buffer salts and contaminants
Applications:
• Protein and DNA desalting/buffer exchange
• Removal of dyes after protein labeling
• Enzyme activity/binding assays
• Drug binding studies (also see Rapid Equilbrium Dialysis 'RED' devices)
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Specifications
3.5 kDa | |
Microdialysis Plate | |
Buffer Exchange | |
Dialysis of 1 to 96 samples, 50–300 μL each | |
Product shipped at ambient temperature. Upon receipt, store at room temperature. |
Cellulose Membrane, Dialysis | |
Microdialysis Plate, 96-well, 0.3 mL, 3.5K MWCO | |
0.3 mL | |
1 Plate Set |
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