Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Protein A/G Plus UltraLink™ Resin

Extremely durable polyacrylamide support with densely immobilized, recombinant Protein A/G for exceptional IgG purification.

Manufacturer:  Thermo Scientific™ 53135

Catalog No. PI53135


Add to cart

Description

Description

Thermo Scientific™ Pierce Protein A/G Plus UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein A/G has been immobilized at high density for use in IgG purification methods.

The Protein A/G Plus UltraLink Resin is used for high-capacity binding and purification of IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A/G is a recombinant fusion protein consisting of the antibody-binding domains of Protein A and Protein G, enabling antibody purification from serum samples of a variety of species.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

  • Immobilized Protein A/G – combines the IgG binding domain of both Protein A and Protein G, enabling purification of numerous IgG subclasses and species

  • High capacity – “Plus” variety has a dense load of immobilized Protein A/G, providing a binding capacity of at least 28mg human IgG per milliliter of resin

  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)
Specifications

Specifications

IgG Antibodies
Protein A/G
Pierce™, UltraLink™
UltraLink™ Resin, Affinity
Resin
Bottle
2 mL
Upon receipt store resin at 4°C. Product is shipped at ambient temperature.
SDS
Documents
Product Certifications
Promotions

Promotions

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit