Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Detect proteins in gels or on membranes via interaction with biotin-labeled bait proteins as primary probes.

Supplier:  Thermo Scientific™ 23500

Catalog No. PI23500

Description

Thermo Scientific™ Pierce Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins via interaction with biotin-labeled test proteins as primary probes.

This far-Western blotting kit provides the reagents and protocol necessary to probe for target proteins by virtue of their interaction with a specific binding partner, which the researcher supplies in purified, biotinylated form. The kit is successful for analysis of protein-protein interactions in which the target (prey) proteins can be bound after they have been separated by gel electrophoresis. The kit includes reagents and procedures for probing and chemiluminescent detection with blots or directly from mini gels.

Highlights:

• On-membrane or in-gel detection options – on-membrane detection offers greater sensitivity; in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer

• Nonradioactive alternative for far-Western analysis – reliable and sensitive biotin/streptavidin-HRP chemistry combined with chemiluminescent detection offers a practical and safe alternative to radiolabeling the bait protein

• Useful interaction range – effective for interaction pairs that have moderate to strong binding associations between the prey and the biotinylated bait protein

• Primary antibody-free detection – uses a biotinylated protein as the probe, eliminating the need for primary antibodies or even to have prior knowledge of target-protein identities

• Compatible with both SDS-PAGE and native gels – provides option to probe for prey proteins in a more native environment because reduced or denaturing systems may not always allow for the intended interaction

• Reduced nonspecific binding – biotin/streptavidin-HRP systems demonstrate less nonspecific binding compared to antibodies directed against the bait protein

• Compatible with protein staining – gels can be used for total protein staining after the in-gel chemiluminescent detection step, eliminating the need to run two gels

Includes:

Streptavidin-HRP, dilution buffer, PBS Packs, stable peroxide and luminol enhancer chemiluminescent substrate, detergent solution and pre-cut cellophane sheets

Specifications

• Product Type: Western Blot Kit
• Product Line: Pierce™
• Reactivity: All species
• Content And Storage: Sufficient For: 10 mini gel or blot experiments with a biotinylated-protein probe
  • Streptavidin-HRP Conjugate, 0.1 mg
  • Dilution Buffer (10X), 50 mL
  • PBS Packs (each makes 500 mL), 17 packs
  • Luminol Enhancer, 55 mL
  • Stable Peroxide Buffer, 55 mL
  • Surfact-Amps 20 Detergent Solution, 6 x 10 mL
  • Pre-Cut Cellophane, 10 sheets
  
  Store kit at 4°C.

• Detection Method: Chemiluminescent
• Substrate: Streptavidin-HRP
• Sufficient For: 10 Mini Gels

For Research Use Only. Not for use in diagnostic procedures.