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Thermo Scientific™ Pierce™ Dextran Desalting Columns (5K MWCO)

Ready-to-use, gel-filtration Thermo Scientific Pierce Dextran Desalting Columns can separate proteins and macromolecules from low-molecular weight buffer salts and reagents up to 5000 Daltons (5 kDa) in 5- or 10-mL volumes.

$180.00 - $202.00

Specifications

Product Type Desalting Column
Content And Storage Store at room temperature or 4°C.
Format Gravity Flow Column
Purification Target Buffer Exchange, Protein
Column Type Size-exclusion, Dextran Resin
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Products 2
Catalog Number Mfr. No. Description Price Quantity & Availability  
Catalog Number Mfr. No. Description Price Quantity & Availability  
PI43230
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Thermo Scientific™
43230
Dextran Desalting Columns, 5K MWCO, 5 mL
Pack of 5 for $180.00
Add to Cart
 
PI43233
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Thermo Scientific™
43233
Dextran Desalting Columns, 5K MWCO, 10 mL
Pack of 5 for $202.00
Add to Cart
 
Description

Description

Thermo Scientific Pierce Dextran Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low molecular-weight buffer salts and reagents.

Applications
• Removing salts from protein solutions
• Eliminating phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Withdrawing unreacted dye from fluorescent antibodies
• Eliminating free radiolabel from labeled proteins
• Buffer exchange

Gravity-flow gel filtration using dextran desalting columns
Gravity-flow gel filtration involves the chromatographic separation of molecules of different dimensions based on their relative abilities to penetrate a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for separation via size exclusion. Different levels of separation can be achieved based on the pore size of the medium packed into the desalting column—in our case, dextran. With size exclusion filtration, large molecules are excluded from the internal pores of the gel and emerge from the column first. Smaller molecules can penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume. Our Dextran Desalting Columns are rigid for easy handling and provide excellent flow properties. Dextran is stable in water, salt solutions, organic solvents and alkaline or weakly acidic solutions. Dextran is also heat-stable and can be autoclaved dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties.

Specifications

Specifications

Desalting Column
Gravity Flow Column
Size-exclusion, Dextran Resin
Pierce™
5 Columns
Store at room temperature or 4°C.
Buffer Exchange, Protein
5.0 kDa
Single-use
SDS
Documents

Documents

Product Certifications
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