Thermo Scientific™ Pierce™ Chicken IgY Purification Kit

Purify chicken antibodies from hen egg yolks with a unique delipidation reagent and precipitation reagent to selectively isolate the IgY fraction.

Supplier:  Thermo Scientific™ 44918

Catalog No. PI44918

Description

Thermo Scientific™ Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.

Highlights:

• Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY

• High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)

• Easy to use – simple precipitation method purifies chicken IgY without affinity columns

• Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date

• Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature

Includes:

Egg separator, delipidation reagent and IgY precipitation reagent

Specifications

• Purification Target: IgY Antibody
• Content And Storage: Sufficient For: Purifying chicken IgY from 5 egg yolks
  • Delipidation Reagent, 500 mL
  • IgY Precipitation Reagent, 500 mL
  • Egg Separator, 1 separator
  
  Store at 4°C.
• Column Type: Precipitation
• Product Line: Pierce™

• Quantity: 1 L
• Description: Pierce Chicken IgY Purification Kit
• Format: Kit, Bottle
• Product Type: IgY Purification Kit