Thermo Scientific™ Pierce™ BCA Protein Assay Kits

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.

Catalog Number	Mfr. No.	Description	Concentration	Kit Contents
PI23225	Thermo Scientific™ 23225	Pierce BCA Protein Assay Kit, 1000 mL	BSA: 2 mg/mL	10 x 1 mL glass ampules
PI23227	Thermo Scientific™ 23227	Pierce BCA Protein Assay Kit, 500 mL	BSA: 2 mg/mL	10 x 1 mL glass ampules
A55864	Thermo Scientific™ A55864	Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards	BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL	4 x 1 mL 8-channel tubestrip, prediluted
A55865	Thermo Scientific™ A55865	Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards	BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL	2 x 1 mL 8-channel tubestrip, prediluted

Description

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip.  The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.

Features of the BCA Protein Assay Kit include:

• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
• Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
• Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
• Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
• Sensitive—detect down to 5 μg/mL with enhanced protocol
• No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve

How the assay works

The BCA Protein Assay combines the well-known reduction of Cu²⁺ to Cu¹⁺ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu¹⁺) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods (i.e., Bradford), the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

Specifications

• Assay: BCA Assay
• Product Line: Pierce™
• For Use With (Application): Solution-based Detection, Absorbance

• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• For Use With (Equipment): Spectrophotometer, Microplate Reader

For Research Use Only.